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KMID : 0380020060210050325
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 5 p.325 ~ p.330
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Microfluidic Immuno-Sensor Chip using Electrical Detection System
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Maeng Joon-Ho
Lee Byung-Chul
Cho Chul-Ho
Ko Yong-Jun
Ahn Yoo-Min
Cho Nahm-Gyoo
Lee Seoung-Hwan
Hwang Seung-Yong
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Abstract
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This study presents the characterization of an integrated portable microfluidic electrical detection system for fast and low volume immunoassay using polystyrene microbead, which are used as immobilization surfaces. In our chip, a filtration method using the microbead was adopted for sample immobilization and immunogold silver staining (IGSS) was used to increase the electrical signal. The chip is composed of an inexpensive and biocompatible Polydimethylsiloxane (PDMS) layer and Pyrex glass substrate. Platinum microelectrodes for electric signal detection were fabricated on the substrate and microchannel and pillar-type microfilters were formed in the PDMS layer. With a fabricated chip, we reacted antigen and antibody according to the procedures. Then, silver enhancer was injected to increase the size of nanogold particles tagged with the second antibody. As a result, microbeads were connected to each other and formed an electrical bridge between microelectrodes. Resistance measured through the electrodes showed a difference of two orders of magnitude between specific and nonspecific immuno-reactions. The detection limit was 10 ng/ml. The developed immunoassay chip reduced the total analysis time from 3 hours to 50 min. Fast and low-volume biochemical analysis has been successfully achieved with the developed microfilter and immuno-sensor chip, which is integrated to the microfluidic system.
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KEYWORD
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Immunoassay, IGSS, nanogold, PDMS, microfilter
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